Page 7 - Spectrum Volume 15 Issue 6

2022 | Spectrum | Volume 15 Number 6 7

Basic steps in qRT-PCR
Kaleidoscope of a Biotechnologist – Revealing patterns, Transforming forever
Rariora 2021 In the world of molecular

JELSIA ALEX constituted Dr R.A. Nazeer (HoD, structure, morphology and testing: qRT-PCR
Department of Biotechnology), Dr laboratory visualization of SHREYA BHAGWATWAR experiment. The steps in RT-
Rariora an annual technical D.V.L. Sarada, Dr S. Subhashini, flagella (locomotory organ). PCR testing are (a) Sample
fest hosted by Anveshna Dr Y. Ravichandran and students - ‘Vibrant Microcosmos’ taught The ongoing pandemic of collection, (b) RNA extraction,
(Biotechnology Association Vamsidhar. A, Sandhya Ramanan, how to differentiate living cells the SARS CoV 2 virus have (c) Reverse transcription, (d) PCR
of SRMIST) to aid students Kirtana Arikath, R. Abdul Raziq. from dead cells. ‘Bioelements illustrated the importance of assay, (e) PCR sequencing. The
from various backgrounds Students from various schools on the Phone’ guided to identify molecular techniques for testing. quantification of the amplicons
to experience fundamental and universities actively took part physicochemical properties The acronym RT-PCR stands for takes place in the final step of
biotechnology at its best was in the workshops. ‘Restriction of genes using bioinformatics Reverse Transcriptase Polymerase PCR sequencing. The purified
conducted on 28th December. Enzyme Digestion’ was one of software. Most importantly, the Chain Reaction. The use of the amplicons are measured using
The three-day technical fest the topics that helped students event illuminated the contributions phrase real-time PCR and reverse spectrophotometry. The current
featured multiple engaging discover how restriction enzymes of biotechnological research to transcriptase PCR interchangeably reports now include a Ct value,
and stimulating workshops for cut DNA. ‘Look at Leukocytes’ medical science in gene regulation, is abundant, but in reality, it is a given according to the assumed
aspiring scientists. The prime provided hands-on learning vaccine development and other combination of the processes, a diagnosis. Ct value (Cycle
mission was to impart knowledge about mature WBCs taken such advancements. Rariora reverse transcription PCR and its Threshold) is the number of
of biology and technology to a from thin blood film and their inspired many young minds quantification. The easiest way amplification cycles needed to
wide range of audiences and and role in improving immunity, to deeply explore the limitless to identify the various variants reach a fixed level of fluorescence
promote cross-domain learning. specifically during the COVID-19 possibilities of biotechnology of the virus is (Nucleic Acid at which the diagnostic results of
The Organizing Committee pandemic. ‘Hanging Microcosmos’ – the impetus to the next big Amplification Test), specific for real-time RT-PCR change from
gave an understanding of the step of mankind! the nucleic acids such as DNA & negative to positive. (Detectable)
RNA found in the virus. Since The current standard for positive
SRMIST Volleyball Women Players - win we will be using PCR, sensitive are Ct levels less than 35. The
Mithilesh only to DNA, the RNA must lower the Ct value, the higher is
gold medal at the 34th Federation Cup be converted into cDNA and the level of infection as Ct values

won Women’s Volleyball Championship then run through the PCR. But vary inversely with logarithmic
quantification becomes a problem concentrations of the nucleic
Gold in as PCR is not a quantification acid targets in the sample.

Equestrian
One day Virtual interaction
Mr.Mithilesh and his Horse
makes SRM Proud in Dream on studies in France
Alliance equestrian challenge.
STAFF WRITER
Mithilesh, student from school
of Law 1st Year B.Com (LLB) The Department of French, CSH, SRMIST conducted a
made SRM IST proud by winning One day Virtual interaction on studies in France. The main
gold medal in the competition purpose of this event was to inform and increase awareness
held at Red Earth Riding school, on the various opportunities available in France. The virtual
Auroville 1st March 2022. interaction had speakers who gave the participants a good
Let us wish him to bring more understanding on how studying in France would look like,
Glory and laurels to SRM IST . the procedures and the necessary steps to be done. The
event was attended by more than 200 students and it was
streamed live on YouTube aswell. The event began with a
quick round of welcome address by Mr. Kumaravel, Asst.
Professor & Head, Department of French and the inaugural
address given by the Dr. A. Duraisamy, Dean, CSH, SRMIST
and felicitated by Dr. Albert Antony Raj. Deputy Dean,
CSH, SRMIST. The chief guest gave valuable insights and
different perspectives of studying abroad. Mr. Eric Perrotel,
Attaché, cooperation for the French Language, who took
us sans frontier the French language. Miss Shruthi took
us through the whole process and giving an overall gist.
The program went for more than an hour. It was a very
productive and informative session with having a few
interactions from some of the students as well.